The freeze-drying of Cowdria ruminantium.
نویسندگان
چکیده
Lyophilized tissues of mice and blood of sheep, infected with either the Kümm, the Welgevonden or the Ball 3 stock of Cowdria ruminantium, remain infective to mice and sheep after storage at 4 degree C for 90 days. Freeze-dried tissues stored at -18 degrees C and -28 degrees C are still infective after 6 months and 2 years, respectively.
منابع مشابه
Identification of drugs effective against Cowdria ruminantium using a mouse screening system.
Heartwater is a tick-transmitted rickettsial infection of ruminants, caused by Cowdria ruminantium. The tetracyclines are the only compounds available for therapy of the disease. A screen, using mice infected with C. ruminantium, was developed and used to identify new compounds with potential for the control of heartwater. A series of di-4-methyl-thiosemicarbazones was shown to be highly active...
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Western blotting (immunoblotting) of Cowdria ruminantium antigens with goat or mouse antiserum identified a periodate-resistant, proteinase K-sensitive immunodominant antigen of 32,000 daltons. This protein, designated Cr32, could be demonstrated in goat choroid plexus infected with one of two different Cowdria stocks. Antisera against nine different Cowdria stocks from Africa and the Caribbean...
متن کاملIdentification of Babesia bovis and Cowdria ruminantium on the island of Unguja, Zanzibar.
Babesia bovis and Cowdria ruminantium were identified for the first time in cattle on Unguja Island, Zanzibar. B bovis is common and widespread, although clinical disease had not been diagnosed previously. The vector of heartwater, Amblyomma variegatum, is found throughout Unguja but C ruminantium appears to be more localised in distribution than B bovis.
متن کاملThe detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum by an enzyme-linked immunosorbent assay.
A sensitive and reliable enzyme-linked immunosorbent assay for the detection of antibodies to Cowdria ruminantium in serum and C. ruminantium antigen in Amblyomma hebraeum nymphae is described. For the screening of antibodies, C. ruminantium from A. hebraeum nymphae, partially purified by wheat-germ lectin affinity chromatography, was used as antigen. To screen nymph populations, sera from eith...
متن کاملThe isolation of nucleic acid sequences specific for Cowdria ruminantium.
Screening of a genomic library of Cowdria ruminantium has yielded twelve clones hybridizing to Cowdria DNA. These clones should be suitable for development into DNA probes specific for this organism.
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ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 57 3 شماره
صفحات -
تاریخ انتشار 1990